hk2 human kidney tubular epithelial cell line  (Procell Inc)

Journal: Renal Failure

Article Title: Isoliquiritigenin attenuates septic acute kidney injury by regulating ferritinophagy-mediated ferroptosis

doi: 10.1080/0886022X.2021.2003208

Figure Lengend Snippet: Measurement of MDA and nitric oxide after ISL treatment upon LPS induction. LPS induce AKI mice models were developed by intraperitoneal ( i.p. ) LPS injection. A total of 30 mice were randomly divided into six groups ( n = 5): control, ISL, Fer, LPS, LPS plus ISL, and LPS plus Fer. An intraperitoneal injection of LPS (10 mg/kg) was made to induce septic AKI. ISL was administered via gavage at 50 mg/kg 30 min before LPS injection. HK2 cells were treated with 50 μM or 100 μM ISL for 5 h, before septic AKI was induced using 2 μg/mL LPS. Cells were collected 24 h after LPS inducing. And the cell experiments were repeated at three times. (A) MDA measurement of mice kidney tissue homogenate. (B) MDA measurement of HK2 cell homogenate. (C) Nitric Oxide Assay of murine serum. (D) Nitric Oxide Assay of HK2 cell supernatant. ‘*’ means compared with the LPS group and p < 0.05. ‘**’ means compared with the LPS group and p < 0.01. ‘***’ means compared with the LPS group and p < 0.001. ‘#’ means compared with the control group and p < 0.05. ‘##’ means compared with the control group and p < 0.01. ‘###’ means compared with the control group and p < 0.001.

Article Snippet: Human kidney epithelial tubular cell line HK2 (ATCC® CRL-2190TM) was grown in DME/F-12 medium (SH30023.01, HyClone, Logan, UT), supplemented with 10% FBS (Gibco, Life Technologies, Lofer, Austria), 100 units/mL penicillin, and 100 units/mL streptomycin (1705694, HyClone, Logan, UT), at 37 °C in a 5% carbon dioxide humidified incubator.

Techniques: Injection, Control, Nitric Oxide Assay

Journal: Renal Failure

Article Title: Isoliquiritigenin attenuates septic acute kidney injury by regulating ferritinophagy-mediated ferroptosis

doi: 10.1080/0886022X.2021.2003208

Figure Lengend Snippet: ISL inhibited Fe 2+ and lipid peroxidation accumulation in LPS-stimulated cells. HK2 cells were treated with 50 μM or 100 μM ISL for 5 h, before septic AKI was induced using 2 μg/mL LPS. Cells were collected 24 h after LPS induction. FerroOrange was used as fluorescent probe to measure the level of Fe 2+ in HK2 cells. Liperfluo was employed to detect the level of lipid peroxidation in HK2 cells. Mito-Ferrogreen could further detect Fe 2+ ions in the mitochondria of HK2 cells. Magnification: ×60 oil.

Article Snippet: Human kidney epithelial tubular cell line HK2 (ATCC® CRL-2190TM) was grown in DME/F-12 medium (SH30023.01, HyClone, Logan, UT), supplemented with 10% FBS (Gibco, Life Technologies, Lofer, Austria), 100 units/mL penicillin, and 100 units/mL streptomycin (1705694, HyClone, Logan, UT), at 37 °C in a 5% carbon dioxide humidified incubator.

Techniques:

Journal: Renal Failure

Article Title: Isoliquiritigenin attenuates septic acute kidney injury by regulating ferritinophagy-mediated ferroptosis

doi: 10.1080/0886022X.2021.2003208

Figure Lengend Snippet: ISL inhibited the expression of HMGB1 and increased the expression of GPX4 both in vivo and in vitro following LPS stimulation. (A) Western blot assay about HMGB1 and GPX4 in murine kidney tissues. (B) Western blot assay about HMGB1 and GPX4 in HK2 cells.

Article Snippet: Human kidney epithelial tubular cell line HK2 (ATCC® CRL-2190TM) was grown in DME/F-12 medium (SH30023.01, HyClone, Logan, UT), supplemented with 10% FBS (Gibco, Life Technologies, Lofer, Austria), 100 units/mL penicillin, and 100 units/mL streptomycin (1705694, HyClone, Logan, UT), at 37 °C in a 5% carbon dioxide humidified incubator.

Techniques: Expressing, In Vivo, In Vitro, Western Blot

Journal: Renal Failure

Article Title: Isoliquiritigenin attenuates septic acute kidney injury by regulating ferritinophagy-mediated ferroptosis

doi: 10.1080/0886022X.2021.2003208

Figure Lengend Snippet: ISL reduced the expression of NOCA4 both in vivo and in vitro upon LPS induction (A) Western blot assay about NCOA4 in murine kidney tissues. (B) Western blot assay about NCOA4 in HK2 cells. (C) Immunohistochemical staining with NCOA4 in mice kidney tissues. Magnification for immunohistochemical staining: ×400.

Article Snippet: Human kidney epithelial tubular cell line HK2 (ATCC® CRL-2190TM) was grown in DME/F-12 medium (SH30023.01, HyClone, Logan, UT), supplemented with 10% FBS (Gibco, Life Technologies, Lofer, Austria), 100 units/mL penicillin, and 100 units/mL streptomycin (1705694, HyClone, Logan, UT), at 37 °C in a 5% carbon dioxide humidified incubator.

Techniques: Expressing, In Vivo, In Vitro, Western Blot, Immunohistochemical staining, Staining

Journal: Central-European Journal of Immunology

Article Title: Resveratrol alleviates sepsis-induced acute kidney injury by deactivating the lncRNA MALAT1/MiR-205 axis

doi: 10.5114/ceji.2021.109195

Figure Lengend Snippet: Primers for genes

Article Snippet: Human kidney proximal tubular epithelial cell 2 (HK2) cell line, purchased from American Type Culture Collection (ATCC, USA), was inoculated in minimum Eagle’s medium (MEM), which contained 10% fetal bovine serum (FBS), 1% penicillin-streptomycin and 1% non-essential amino acids (NEAA), under an atmosphere of 37°C and 5% CO 2 .

Techniques:

Journal: Central-European Journal of Immunology

Article Title: Resveratrol alleviates sepsis-induced acute kidney injury by deactivating the lncRNA MALAT1/MiR-205 axis

doi: 10.5114/ceji.2021.109195

Figure Lengend Snippet: Resveratrol reduced proliferation and inflammatory response of HK2 cells by regulating lncRNA MALAT1 and miR-205. A ) MALAT1 expression was determined in HK2 cells of PBS group, LPS group and LPS + Res group. *** p < 0.001. B ) MALAT1 expression in HK2 cells was altered after transfection of si-MALAT1 and pcDNA3.1- MALAT1. *** p < 0.001. C ) Viability of HK2 cell line was determined after treatments of PBS, LPS, LPS + Res, LPS + si-MALAT1 and LPS + Res + pcDNA3.1-MALAT1. *** p < 0.001 in comparison to PBS group; ### p < 0.001 in comparison to LPS group; @@@ p < 0.001 in comparison to LPS + Res group. D-F ) TNF-α (D), IL-1β (E) and IL-6 (F) levels were compared among PBS group, LPS group, LPS + Res group, LPS + si-MALAT1 group and LPS + Res + pcDNA3.1-MALAT1 group. *** p < 0.001 in comparison to PBS group; ### p < 0.001 in comparison to LPS group; @@@ p < 0.001 in comparison to LPS + Res group. G ) MiR-205 expression in HK2 cells was measured after transfection of si-MALAT1 and pcDNA3.1-MALAT1. *** p < 0.001. H) MiR-205 expression in HK2 cells was compared among PBS group, LPS group, LPS + Res group, LPS + si-MALAT1 group and LPS + Res + pcDNA3.1-MALAT1 group. *** p < 0.001 in comparison to PBS group; ### p < 0.001 in comparison to LPS group; @@@ p < 0.001 in comparison to LPS + Res group

Article Snippet: Human kidney proximal tubular epithelial cell 2 (HK2) cell line, purchased from American Type Culture Collection (ATCC, USA), was inoculated in minimum Eagle’s medium (MEM), which contained 10% fetal bovine serum (FBS), 1% penicillin-streptomycin and 1% non-essential amino acids (NEAA), under an atmosphere of 37°C and 5% CO 2 .

Techniques: Expressing, Transfection, Comparison

Journal: The Journal of Biological Chemistry

Article Title: Notch Pathway Is Activated via Genetic and Epigenetic Alterations and Is a Therapeutic Target in Clear Cell Renal Cancer *

doi: 10.1074/jbc.M116.745208

Figure Lengend Snippet: Integrative analysis reveals that JAG1 and JAG2 are associated with DNA hypomethylation and gene amplification in CCRCC. A, loss of DNA methylation affecting the JAG1 gene locus was seen in CCRCC samples when compared with controls. Differentially hypomethylated intragenic regions in CCRCC samples for the JAG1 locus overlap with kidney specific H3K4me1 enhancer peaks. Methylation values generated by the HELP assay (log(HpaII/MspI) are shown as peaks (positive values corresponding with less methylation are shown in black, and negative values corresponding to increased methylation are shown in gray). Histone modification peaks are shown for adult kidney (BI27). Chromatin occupancy for other tissue types are shown at the bottom. The legend shows color coding for different regions with yellow regions representing enhancers. GM12878, lymphoblastoid cell; H1-hESC, embryonic stem cells; K562, leukemic cells; HepG2, hepatic cells; HUVEC, umbilical vein endothelial cells; HMEC, mammary epithelial cells; HSMM, skeletal muscle myoblasts; NHEK, epidermal keratinocytes; NHLF, lung fibroblasts. B, MassARRAY EpiTYPER analysis shows quantitative hypomethylation in CCRCC samples (n = 6) when compared with healthy controls (n = 6). C, treatment of non-transformed renal tubular cell lines HK2 and HKC8 with decitabine (DAC) results in increased expression of JAG1 by qRT-PCR (means ± S.E. of three independent experiments, P < 0.05, t test). D, MassARRAY EpiTYPER analysis of the JAG1 locus shows decrease in methylation after DAC treatment (means ± S.E. of three independent experiments, t test; P < 0.05 (*)). E, JAG2 lies on chromosome 14 (14q32). MspI representation identified 3 of 13 samples with JAG2 amplification. Representative sample is shown with JAG2 amplification indicated by the arrows.

Article Snippet: Normal human kidney tubular epithelial cell line HK2 and renal cell cancer cell lines 786-O, 769-P, and Caki were purchased from ATCC (with authentication).

Techniques: Amplification, DNA Methylation Assay, Methylation, Generated, HELP Assay, Modification, Transformation Assay, Expressing, Quantitative RT-PCR